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Image Search Results


a , Schematic overview of the mouse Apc gene, 20 amino acid (AA) β-catenin binding sites are labelled in green and sgRNA target sites are marked with arrows. b , Schematic illustration of the TOP-GFP Wnt reporter construct. c , Mutation-dependent upregulation of Wnt signalling as measured by GFP expression (n=5). d, e , Axin2 ( d ) and Notum ( e ) expression in mouse embryonic fibroblast (MEF) β-catenin binding site cell lines and control lines (n=3). f, g , Schematic illustration of proximal small intestinal wild-type organoids targeted with CRISPR/Cas9 to introduce specific alterations in the number of β-catenin binding sites ( f ) and representative phase images of transduced organoids ( g ). h, i , Relative Axin2 ( h ) and Notum ( i ) expression in β-catenin binding site organoid lines and control lines (n=3).

Journal: bioRxiv

Article Title: Apc truncation enforces optimal Wnt signalling during early intestinal tumourigenesis

doi: 10.64898/2026.02.11.705236

Figure Lengend Snippet: a , Schematic overview of the mouse Apc gene, 20 amino acid (AA) β-catenin binding sites are labelled in green and sgRNA target sites are marked with arrows. b , Schematic illustration of the TOP-GFP Wnt reporter construct. c , Mutation-dependent upregulation of Wnt signalling as measured by GFP expression (n=5). d, e , Axin2 ( d ) and Notum ( e ) expression in mouse embryonic fibroblast (MEF) β-catenin binding site cell lines and control lines (n=3). f, g , Schematic illustration of proximal small intestinal wild-type organoids targeted with CRISPR/Cas9 to introduce specific alterations in the number of β-catenin binding sites ( f ) and representative phase images of transduced organoids ( g ). h, i , Relative Axin2 ( h ) and Notum ( i ) expression in β-catenin binding site organoid lines and control lines (n=3).

Article Snippet: Mouse embryonic fibroblasts (MEFs, ATCC) were cultured in DMEM (Gibco), supplemented with 10% FCS, 1% glutamine and an antibiotic penicillin-streptomycin solution.

Techniques: Binding Assay, Construct, Mutagenesis, Expressing, Control, CRISPR, Introduce